Anther Culture of Pepper (capsicum Annuum L.) : Comparative Study on Effect of the Genotype

There were investigated and compared the in vitro response in anther culture of 16 Bulgarian pepper (Capsicum annuum L.) genotypes: six lines, six varieties and four hybrids developed in Maritsa Vegetable Crops Research Institute, Plovdiv. The anthers reacted with induction of direct embryogenesis or callusogenesis. Only in two of the studied genotypes (line N145 and variety Zlaten medal) the anthers have shown indirect organogenesis. There were established genotypes with good embryogenic ability – varieties Zlaten medal, Albena, Stryama, Hebur, Kourtovska kapiya, F1 hybrids N1647Χ N668, N1647 Χ N1962 and lines N1312, N145 and N668. The embryoids obtained from anthers of line N145 and varieties Stryama and Kourtovska kapiya developed to regenerants with formed roots and cotyledons. The results from this study proved that for the first time is achieved a successful induction of embryogenesis in vitro and obtaining plant-regenerants, with microspore origin, of Bulgarian pepper genotypes.